Paper-based molecular diagnostic for Chlamydia trachomatis

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Paper-based molecular diagnostic for Chlamydia trachomatis.

Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point of care detection of sexually transmitted infections caused by Chlamydia trachomatis. This new diagnostic platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual detection using only a pressure source and heat block, eliminating the need for expensive la...

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Chlamydia trachomatis: Molecular Testing Methods

Screening tests to detect Chlamydia trachomatis infections have advanced to permit the detection of nucleic acids from organisms. The development of these molecular assays that can detect and amplify the specific nucleic acids from the genes of C. trachomatis are called nucleic acid amplification tests (NAATs) and they have expanded the range of available specimen types to include noninvasive s...

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Diagnostic Procedures to Detect Chlamydia trachomatis Infections

The intracellular life style of chlamydia and the ability to cause persistent infections with low-grade replication requires tests with high analytical sensitivity to directly detect C. trachomatis (CT) in medical samples. Nucleic acid amplification tests (NAATs) are the most sensitive assays with a specificity similar to cell culture and are considered the method of choice for CT detection. In...

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Molecular Detection of Ureaplasma urealyticum and Chlamydia trachomatis in Women with Endometriosis

Background and Aims: Endometriosis is considered to be one of the most common women's diseases in the world. The exact cause of endometriosis is unknown. In this study, the bacterial agents were considered as the probable factors influencing this disease and the frequency of two bacteria U. urealyticum and C. trachomatis was studied in this disease. Materials and Methods: This study was perfor...

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Screening for Chlamydia trachomatis among Infertile Women Using PCR Method

  Background & objectives: Chlamydia trachomatis is a gram negative bacterium and chlamydia infection, as a curable infection, is one of the most common sexually transmitted diseases (STD). With regard to the essential role of chlamydia in infertility, the study of the prevalence of asymptomatic cases is precious. The aim of this study was to determine of the prevalence of chlamydia trachomatis...

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ژورنال

عنوان ژورنال: RSC Adv.

سال: 2014

ISSN: 2046-2069

DOI: 10.1039/c4ra07911f